Study title
Metabolic Profiling Reveals Differences in Concentrations of Oxylipins and Fatty Acids Secreted by the Infrapatellar Fat Pad of Donors With End-Stage Osteoarthritis and Normal Donors
Template
Intervention/Observation study
title
Metabolic Profiling Reveals Differences in Concentrations of Oxylipins and Fatty Acids Secreted by the Infrapatellar Fat Pad of Donors With End-Stage Osteoarthritis and Normal Donors
description
Objective. The infrapatellar fat pad (IPFP) in the
knee joint is hypothesized to contribute to osteoarthritis
(OA) development possibly by influencing inflammatory
processes. Oxylipins are essential mediators in the
inflammatory process. We undertook this study to identify
the secretion of fatty acids and oxylipins derived
thereof by the IPFP.
Methods. IPFP explants from 13 OA donors
undergoing joint replacement surgery and from 10
normal donors postmortem were cultured for 24 hours,
and supernatants (fat-conditioned medium [FCM])
were collected. Liquid chromatography tandem mass
spectrometry detected fatty acids and oxylipins in FCM
samples. Univariate and multivariate (partial leastsquares
discriminant analysis [PLS-DA]) analyses were
performed followed by pathway analysis. To validate
these outcomes, a second set of OA FCM samples was
measured (n 23).
Results. Twenty-nine oxylipins and fatty acids
could be detected in FCM. Univariate analysis showed
no differences between normal donor and OA donor
FCM; however, PLS-DA revealed an oxylipin/fatty acid
profile consisting of 14 mediators associated with OA
(accuracy rate 72%). Most important contributors to the
model were lipoxin A4 (decreased), thromboxane B2
(increased), and arachidonic acid (increased). The statistical
model predicted 64% of the second set of OA
FCM samples correctly. Pathway analysis indicated
differences in individual mediators rather than in complete
pathways.
Conclusion. The IPFP secretes multiple and different
oxylipins, and a subset thereof provides a distinctive
profile for OA donors. The observed changes are
likely regulated by the OA process rather than a consequence
of basal metabolism changes, as an increase in
fatty acid levels was not necessarily associated with an
increase in oxylipins derived from that fatty acid.
startDate
2013-08-15 00:00:00.0
Study type
Observational Human
Objectives
Objective. The infrapatellar fat pad (IPFP) in the
knee joint is hypothesized to contribute to osteoarthritis
(OA) development possibly by influencing inflammatory
processes. Oxylipins are essential mediators in the
inflammatory process. We undertook this study to identify
the secretion of fatty acids and oxylipins derived
thereof by the IPFP.
