Study title
PPS1 - Development of insulin resistance on a high fat diet in ApoE3 mice
Template
Intervention/Observation study
title
PPS1 - Development of insulin resistance on a high fat diet in ApoE3 mice
description
Male ApoE3Leiden transgenic mice exhibiting a humanized lipid metabolism were fed a high-fat diet (HFD) for 0, 1, 6, 9, or 12 weeks. Development of IR was monitored in individual mice over time by performing glucose tolerance tests and measuring specific biomarkers in plasma, and hyperinsulinemic-euglycemic clamp analysis to assess IR in a tissue-specific manner. To elucidate the dynamics and tissue-specificity of metabolic and inflammatory processes key to IR development, a time-resolved systems analysis of gene expression (Affymetrix arrays) and metabolite levels (free fatty acids LC-MS, GCxGC-MS) in liver, white adipose tissue (WAT), and muscle was performed.
startDate
2006-07-25 00:00:00.0
Study type
Animal Intervention
Objectives
To evaluate the acute metabolic stress responses to a dietary challenge (bolus of glucose) over time in C57BL/6 mice fed a 10% low fat or a 45% high fat diet for 12 weeks. More specifically: a) to compare the whole body response to a metabolic stressor (glucose in a GTT) under two conditions that reflect a ‘healthy status’ (low-fat dietary conditions) and ‘insulin resistance’ (high-fat dietary conditions), respectively. b) to generate a time-resolved, well-powered OMICs dataset in which the acute response of a ‘healthy’ organism and an “insulin-resistant” organism to a metabolic stressor (glucose in a GTT) will be assessed in several tissues including plasma, liver and adipose tissue. Centres involved TNO, UCD, INRA, RRI, IFR.
Institute
Centres involved: Oslo U., TNO, TUM, Wageningen U., Maastricht U., Ancona U.
