Study title
PPS2 - Evaluation of the acute whole-body response to glucose stress in C57BL/6J mice
Template
Intervention/Observation study
title
PPS2 - Evaluation of the acute whole-body response to glucose stress in C57BL/6J mice
description
Mice with an isogenic background (C57BL/6J mice) were fed diets for 12 weeks containing either 10 or 45% fat by energy (mainly palm oil) after 2 weeks of adaptation to laboratory conditions with a control diet. Plasma was collected throughout the 12 weeks of feeding to monitor the development of insulin resistance establishing that animals on the high fat diet had developed insulin resistance after 3 months. Healthy and insulin resistant animals were subjected to a glucose tolerance test at the end of the feeding trial followed by comprehensive phenotyping. The core of the study was performed at TNO (coordinated by R.K. and T.K.). Serum, liver and selected tissues like muscle and adipose tissue were removed 0 min, 40 min, 2, 18 and 48 h after the glucose challenge. Glucose was injected via the i.p. route at 1 p.m. in all groups and animals had free access to feed during the challenge (no fasting). The liver will be analyzed by transcriptomics, and by proteomic and metabolomic approaches. Metabolomic analysis is also being performed in muscle, another tissue affected by insulin resistance, whilst serum is being analyzed by both proteomics and metabolomics. Moreover, the collaborations mentioned in PPS1 have been established here also to analyze the impact of this metabolic challenge on colonic tissues.
startDate
2007-02-01 00:00:00.0
Study type
Animal Intervention
Objectives
To evaluate the acute metabolic stress responses to a dietary challenge (bolus of glucose) over time in C57BL/6 mice fed a 10% low fat or a 45% high fat diet for 12 weeks. More specifically: a) to compare the whole body response to a metabolic stressor (glucose in a GTT) under two conditions that reflect a ‘healthy status’ (low-fat dietary conditions) and ‘insulin resistance’ (high-fat dietary conditions), respectively. b) to generate a time-resolved, well-powered OMICs dataset in which the acute response of a ‘healthy’ organism and an “insulin-resistant” organism to a metabolic stressor (glucose in a GTT) will be assessed in several tissues including plasma, liver and adipose tissue.
Institute
Mouse study performed by TNO.
Centres involved TNO, UCD, INRA, RRI, IFR
